tRNA in vitro production Oligonucleotides containing tRNA under a T7 promoter and followed by an HDV ribozyme61 were synthesized by IDT as high-fidelity DNA microchip oligo pools from 254–275 nucleotides in length, and dsDNA was amplified in a single PCR reaction for each pool. These tRNA pools included 48 unique E. coli isoacceptor tRNAs with
The Dutch Data Protection Authority is fining Uber €825 million (around $966 million) — the second largest penalty issued so far under Europe’s General Data Protection Regulation, according to Reuters. The Dutch regulator was investigating complaints that Uber had deactivated driver accounts through an automated process without sufficient warning or human oversight. In a statement,
