Cell culture HCT116 cells and derivative cell lines (Supplementary Table 1) were grown in DMEM (Life Technologies, 41966-029) supplemented with 10% fetal bovine serum (FBS, GIBCO), 100 U ml−1 penicillin and 100 mg ml−1 streptomycin in an atmosphere containing 6% CO2 at 37 °C. HCT116 OsTIR (F74G), mAID-mClover-RAD21 HCT116 OsTIR (F74G)6, WAPL-mClover-mAID HCT116 OsTIR (F74G)4 and CTCF-mClover-mAID HCT116 OsTIR (F74G)6
Simmel, F. C. in Visions of DNA Nanotechnology at 40 for the Next 40: A Tribute to Nadrian C. Seeman (eds Jonoska, N. & Winfree, E.) 17–29 (Springer, 2023). Wolpert, D. H. The stochastic thermodynamics of computation. J. Phys. A Math. Theor. 52, 193001 (2019). Article ADS MathSciNet Google Scholar Doty, D., Rogers, T. A.,
Clinical trial design, patients and conduct The Algonquin study is an ongoing, multicentre, open-label, single-arm, two-part study (ClinicalTrials.gov identifier: NCT03715478)) of BPd in patients with relapsed/refractory MM. Part 1 of the trial consisted of a dose-exploration phase22. Part 2 is evaluating the safety, tolerability and clinical activity of the dose and schedule identified in part 1. Patients included
Hello Nature readers, would you like to get this Briefing in your inbox free every day? Sign up here. Mapping microproteins in the brain might help to understand, and treat, Alzheimer’s disease.Credit: Zephyr/SPL More than 1,000 previously overlooked ‘microproteins’ have been found in samples of human brain tissue from people with and without Alzheimer’s disease.
Scientists spend a lot of time ordering things: laboratory equipment, samples and consumables. What if a researcher’s to-do list included ordering an antimatter delivery? In March, the BASE Collaboration brought that reality one step closer. Writing in Nature, Leonhardt et al. sealed 92 antiprotons in a specialized ultrahigh-vacuum trap, loaded them into a truck and
Explanation: Also known as the false dawn, a luminous band of zodiacal light is captured in this dark night skyscape. The serene view was recorded just before the beginning of astronomical twilight during September’s star party at the remote Hanle Dark Sky Reserve, Ladakh, India, planet Earth. At about 4,500 meters altitude, the dark sky