My bedroom has become my escape, my sanctuary, my necessary respite from the hustle and bustle of a big family, including four kids and four rescue dogs. When I’m in my room, and the door is closed, my family knows there are very few reasons why they would be allowed to disturb me. Now, before
In my 20s, I fell in love with my partner’s ambition. His dream was to own a restaurant, and I admired his willingness to reject a traditional 9-to-5 to build something of his own. He entered the industry as a teenager, worked his way up to general manager at a local restaurant by his mid-20s,
Cell culture MCF7 (HTB-22), ZR-75-1 (CRL-1500), CAMA-1 (HTB-21), MDA-MB-453 (HTB-131), MDA-MB-231 (HTB-26), OVCAR-3 (HTB-161) and Kuramochi (JCRB0098) cells were obtained from the American Type Culture Collection. MCF7 ESR1Y537S cells were provided by S. Chandarlaparty35. MCF7, ZR-75-1, MDA-MB-231 and CAMA-1 cells were cultured in RPMI-1640 medium (11875-093, Gibco) supplemented with 10% fetal bovine serum (FBS; SH30054.03,
