RNA sequencing and analysis E. coli BL21(DE3) cells (NEB: C2527H), which lack endogenous copies of IS621 elements, were transformed with plasmids encoding either the RE–LE junction or the LE–recombinase–RE region of the IS621 element. The cells were plated on LB (lysogeny broth) agar with kanamycin and grown overnight at 37 °C. Colonies were scraped from the
Singapore’s bourse reported a 14% jump in yearly revenue to $1.17 billion, after the country rolled out a series of market reforms in 2025 aimed at revitalizing its sluggish stock market. The measures, which included tax rebates for newly-listed companies and a government-led injection of 1.5 billion Singapore dollars into the local equity market, have
Traffic will begin to flow across a new bridge over the Detroit River that will serve as a critical route for commerce, jobs and tourism between Canada and the U.S. Last week, Canada celebrated the Gordie Howe International Bridge with a ribbon cutting… DETROIT– Traffic will begin flowing Monday across a new bridge over the
Almost 15 years ago, Canada agreed to pay in full for the bridge’s construction to overcome American political resistance and get the project underway, seen as necessary infrastructure to solve a trade bottleneck. The bridge will help support more than C$1 billion (US$710 million; £530 million) of freight passing through the Windsor-Detroit crossing each day.
DETROIT– The tense relationship between the United States and Canada is ruining the celebration of an extraordinary project between the two countries: a new six-lane bridge named after a famous Canadian who played hockey for the Detroit Red Wings. After years of planning and billions of dollars in construction, the Gordie Howe International Bridge is
The former chief executive of Italy’s motorway operator is among 32 people found guilty over the August 2018 collapse of Genoa’s Morandi Bridge in a trial. Giovanni Castellucci, already serving a six-year prison sentence for a 2013 road disaster, received a 12-year sentence for the disaster. Forty-three people died when the highway bridge through the