Cell culture (University College London) Key reagents can be found in Supplementary Table 1. SH-SY5Y cells (American Type Culture Collection (ATCC)) and SK-N-BE(2) (ATCC) with stable doxycycline-inducible short-hairpin RNA (shRNA) to TARDBP were described in ref. 6 and were maintained in DMEM–F12 with GlutaMAX (Thermo Fisher Scientific) with 10% fetal bovine serum (FBS) (Thermo Fisher
Ethics statement All experiments involving human cell lines were approved by the Harvard University IRB and ESCRO committees. All animal experiments were conducted according to protocols approved by the Institutional Animal Care and Use Committee (IACUC) of Harvard University. All experiments were performed in accordance with relevant guidelines and regulations, and in accordance with informed
Mouse models All animal experiments at the University of California Los Angeles (UCLA) were conducted in accordance with the US National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals and were approved by the Chancellor’s Animal Research Committee at UCLA. The UCLA ARC protocol numbers for the experiments were 2009-043, 2020-002, 2017-039
