Plant material Four sets of A. thaliana materials were analysed in this study, including MA16 and MA32 lines, HPG1 accessions, and rtel1-1 mutants (Supplementary Table 1). The MA16 lines (two lines, samples A and B) were derived from a trans-generational mutation accumulation experiment, originating from a single Col-0 mother plant (Nottingham Arabidopsis Stock Centre ID