KD with CasRx CasRx17, specifically NLS-RfxCas13d-NLS (Addgene, 109049), was used in combination with target-specific gRNAs for KD of targeted transcripts. A deep-learning-based tool66 was used to design gRNA spacer sequences targeting the genes of interest. These spacer sequences were filtered to exclude homopolymers (more than four As, more than three Cs, Gs or Ts). Spacer
