Mice C57BL/6, P14 TCR-transgenic (Tg)45, pmel TCR-Tg46 and Rosa26-Cas9 knock-in47 mice were purchased from the Jackson Laboratory. Human CD19 CAR-Tg mice were provided by T. Geiger36. We crossed Rosa26-Cas9 knock-in mice with P14, pmel or human CD19 (hCD19) CAR-Tg mice to express Cas9 in antigen-specific CD8+ T cells. The Cas9-expressing mice were fully backcrossed to
