MPRA design Library design To generate the catalogue of fixed human-derived substitutions we used genotyping data from 139 non-human great apes15,68,69,70,71 (10 bonobos, 59 chimpanzees, 43 gorillas and 27 orangutans), all mapped to the human reference genome GRCh37. To restrict the analysis to high-confidence genotypes, we excluded variants that did not meet any of the
Plasmids and inserts Sequences and accompanying information are given in Supplementary Tables 2–4. In brief, we selected Codebook TFs (and their DBDs) from information published in a previous study1 and posted at https://humantfs.ccbr.utoronto.ca. Inserts named with a ‘-FL’ suffix correspond to the full-length ORF of a representative isoform of the protein. Those with a ‘-DBD’
