DNA sequencing WGS and WXS data were newly generated for the MD Anderson Cancer Center, Duke University Hospital, Henry Ford Hospital, Case Western Reserve University, Chinese University of Hong Kong, Mayo Clinic, Northwestern Medicine, Olivia Newton-John Cancer Research Institute, University of California, San Francisco, Saint Joseph’s Hospital and Medical Center, Seoul National University and Luxembourg
Cell culture and nucleofection K562 (CCL-243) and Jurkat (TIB-152) cells were obtained from American Type Culture Collection (ATCC) and cultured at 37 °C under 5% CO2 in RPMI media (ThermoFisher Scientific, 11875093) supplemented with 10% FBS, and 1% Penicillin/Streptomycin (ThermoFisher Scientific, 15140122). HEK293T (CRL-1573) cells were obtained from the ATCC and cultured at 37 °C under 5%
Atahualpa, the last Incan emperor, was seized by troops in 1532 during the Spanish Conquest, but smallpox had already devastated the Inca Empire around 1524–1528.Credit: Florilegius/Alamy More than 400 years ago, two people living in modern day Chile died while infected with the smallpox virus. Viral DNA found in their mummified remains has now yielded
