Schneider, J. L., Lin, J. J. & Shaw, A. T. ALK-positive lung cancer: a moving target. Nat Cancer 4, 330–343 (2023). Article CAS PubMed PubMed Central Google Scholar Voena, C., Ambrogio, C., Iannelli, F. & Chiarle, R. ALK in cancer: from function to therapeutic targeting. Nat. Rev. Cancer 25, 359–378 (2025). Article CAS PubMed PubMed
Expression and purification of β2AR in Sf9 cells The β2AR construct PN1 was expressed and purified as previously described39,42. In brief, Sf9 cells were infected with a PN1-containing baculovirus produced using the BestBac method. Cells were then collected and resuspended in chilled lysis buffer containing 10 mM HEPES, pH 7.4, 1 mM EDTA, 1 μM alprenolol and protease inhibitors
Compounds dBET6 (HY-112588), dBRD9 (HY-117690), MLN7243 (HY-100487), MLN4924 (HY-70062), MG132 (HY-13259), bafilomycin A1 (HY-100558), and 5K Scaffold Library (HY-L902) were obtained from MedChemExpress. Plasmids The following plasmids were used in this study: Artichoke (Addgene #73320) and Cilantro (Addgene #74450) for flow-based reporter degradation assays, reporter CRISPR screens and co-immunoprecipitation; sgBFP (U6.sgRNA.SFFV.tBFP) for validation of DCAF11-knockout
