Human tissue and ethical approval Post-mortem human brain specimens were obtained from the Department of Neuroscience at Yale University School of Medicine, the Birth Defects Research Laboratory at the University of Washington, Advanced Bioscience Resources (ABR), the Human Brain Collection Core (HBCC), the Brain and Tissue Bank at the University of Maryland, the MRC–Wellcome Trust
RNA sequencing and analysis E. coli BL21(DE3) cells (NEB: C2527H), which lack endogenous copies of IS621 elements, were transformed with plasmids encoding either the RE–LE junction or the LE–recombinase–RE region of the IS621 element. The cells were plated on LB (lysogeny broth) agar with kanamycin and grown overnight at 37 °C. Colonies were scraped from the
