Inhibitors for in vitro and cell-based assays were obtained as follows: ponatinib (MedChemExpress (MCE), HY-12047); asciminib (MCE, HY-104010); imatinib (MCE, HY-15463); dasatinib (MCE, HY-10181); osimertinib (MCE, HY-15772); and RMC-5552 (MCE, HY-132168). DasatiLink-1 and asc-tracer were obtained from the same batches as previously described14,34. DAS-DFGO-II and DAS-CHO-II were provided by M. Soellner. Das-tracer44 was provided by Promega. Synthesis
Bacterial strains All strains for the experiments were grown in lysogeny broth (LB) Lennox and the required antibiotics and incubated at 37 °C with shaking. E. coli K-12 (BW25113)72 was used for most biological analyses, and the BL21-AI or NEB5α strains were used for protein purification and cloning, respectively. The use of the ECOREF53 strain collection
