Bacterial strains All strains for the experiments were grown in lysogeny broth (LB) Lennox and the required antibiotics and incubated at 37 °C with shaking. E. coli K-12 (BW25113)72 was used for most biological analyses, and the BL21-AI or NEB5α strains were used for protein purification and cloning, respectively. The use of the ECOREF53 strain collection