Mice All mice were group-housed and experiments were conducted in accordance with procedures approved by the Institutional Animal Care and Use Committees at the Massachusetts General Hospital and the Tufts University School of Medicine and NIH guidelines. All mice were housed in a 12-h (7:00 to 19:00) light–dark colony room at 22–24 °C, 30–50% humidity and
Patient biospecimens and data All patient material was obtained through Memorial Sloan Kettering (MSK) Institutional Review Board protocols 06-107, 12-245, 14-244 and 22-404. No statistical method was used to predetermine sample sizes. scRNA-seq datasets from matched normal colon, primary tumour and metastasis samples were obtained from a previous study10. Patient-derived organoids from two primary tumours
