Plant material Plants of R. tenuis and R. austrobrasiliensis were collected from natural populations in Brazil under appropriate permits and cultivated under controlled greenhouse conditions. Nine geographically distinct accessions of R. tenuis were used for genome sequencing and cytological analyses. Individual flowers and pollen were staged for meiotic analyses. All experimental material was propagated clonally
Study population and ethics declaration The MCPS is a prospective cohort of over 150,000 adult participants from Mexico City3,4. The baseline survey took place between 14 April 1998 and 28 September 2004 and focused on households in two urban districts, Coyoacán and Iztapalapa. Residents aged 35 years or older were invited to participate in the study. Of
The Norwegian MoBa cohort MoBa is a population-based pregnancy cohort study conducted by the Norwegian Institute of Public Health. Participants were recruited from all over Norway from 1999 to 2008. The women consented to participation in 41% of the pregnancies (n = 112,908 recruited pregnancies)43,54. The cohort includes approximately 114,500 children, 95,200 mothers and 75,200 fathers. The
Pangenome reference The pangenome reference used in this study is a sequence graph augmented with phased small variants. It is stored in two data files: a sequence graph in reference rGFA format16 containing the structural variants and a supplementary phased VCF33 file containing small variants. The rGFA sequence graph is constructed first from haplotype assemblies,
