Cohesin reshapes replication fork contacts to aid fork slowing and reversal – Nature0

• SCIENCE, Science / Health, Science Mission Directorate, scientists, Space, Space Communications & Navigation Program, Space Launch System (SLS), Space Telescope, space-x-ipo, SpaceX
• September 19, 2026

Cell culture HCT116 cells and derivative cell lines (Supplementary Table 1) were grown in DMEM (Life Technologies, 41966-029) supplemented with 10% fetal bovine serum (FBS, GIBCO), 100 U ml−1 penicillin and 100 mg ml−1 streptomycin in an atmosphere containing 6% CO2 at 37 °C. HCT116 OsTIR (F74G), mAID-mClover-RAD21 HCT116 OsTIR (F74G)6, WAPL-mClover-mAID HCT116 OsTIR (F74G)4 and CTCF-mClover-mAID HCT116 OsTIR (F74G)6