DNA sequencing WGS and WXS data were newly generated for the MD Anderson Cancer Center, Duke University Hospital, Henry Ford Hospital, Case Western Reserve University, Chinese University of Hong Kong, Mayo Clinic, Northwestern Medicine, Olivia Newton-John Cancer Research Institute, University of California, San Francisco, Saint Joseph’s Hospital and Medical Center, Seoul National University and Luxembourg
Selection and prioritization of extracellular tumour-derived genes Candidate extracellular tumour-derived genes were identified using a stepwise filtering and prioritization strategy integrating single-cell transcriptomic analyses, protein subcellular localization annotations, functional dependency data, domain-based functional annotation and literature-based curation (Extended Data Fig. 1a). Publicly available scRNA-seq datasets from human14 and mouse15 PDAC were independently analysed. Within each
