Freitas, F. & Attwell, D. Pericyte-mediated constriction of renal capillaries evokes no-reflow and kidney injury following ischaemia. eLife 11, e74211 (2022). Article CAS PubMed PubMed Central Google Scholar O’Farrell, F. M. & Attwell, D. A role for pericytes in coronary no-reflow. Nat. Rev. Cardiol. 11, 427–432 (2014). Article PubMed Google Scholar Rezkalla, S. H. &
Freitas, F. & Attwell, D. Pericyte-mediated constriction of renal capillaries evokes no-reflow and kidney injury following ischaemia. eLife 11, e74211 (2022).
Google Scholar
O’Farrell, F. M. & Attwell, D. A role for pericytes in coronary no-reflow. Nat. Rev. Cardiol. 11, 427–432 (2014).
Google Scholar
Rezkalla, S. H. & Kloner, R. A. No-reflow phenomenon. Circulation 105, 656–662 (2002).
Google Scholar
O’Farrell, F. M. et al. Capillary pericytes mediate coronary no-reflow after myocardial ischaemia. eLife 6, e29280 (2017).
Google Scholar
Pénzes, M. et al. Direct myosin-2 inhibition enhances cerebral perfusion resulting in functional improvement after ischemic stroke. Theranostics 10, 5341–5356 (2020).
Google Scholar
Kloner, R. A., Ganote, C. E. & Jennings, R. B. The “no-reflow” phenomenon after temporary coronary occlusion in the dog. J. Clin. Invest. 54, 1496–1508 (1974).
Google Scholar
Lameire, N. H. et al. Acute kidney injury: an increasing global concern. Lancet 382, 170–179 (2013).
Google Scholar
Mehta, R. L. et al. International Society of Nephrology’s 0by25 initiative for acute kidney injury (zero preventable deaths by 2025): a human rights case for nephrology. Lancet 385, 2616–2643 (2015).
Google Scholar
Gibson, C. M. et al. Relationship of TIMI myocardial perfusion grade to mortality after administration of thrombolytic drugs. Circulation 101, 125–130 (2000).
Google Scholar
Ndrepepa, G. et al. Coronary no-reflow and adverse events in patients with acute myocardial infarction after percutaneous coronary intervention with current drug-eluting stents and third-generation P2Y12 inhibitors. Clin. Res. Cardiol. 113, 1006–1016 (2024).
Google Scholar
Zhang, B. et al. The coronary angiography-derived index of microcirculatory resistance predicts perioperative myocardial injury in stable coronary artery disease patients undergoing PCI. Heliyon 10, e35240 (2024).
Google Scholar
Nicolau, A. M. et al. Molecular mechanisms of microvascular obstruction and dysfunction in percutaneous coronary interventions: from pathophysiology to therapeutics—a comprehensive review. Int. J. Mol. Sci. 26, 6835 (2025).
Google Scholar
Peppiatt, C. M., Howarth, C., Mobbs, P. & Attwell, D. Bidirectional control of CNS capillary diameter by pericytes. Nature 443, 700–704 (2006).
Google Scholar
Yemisci, M. et al. Pericyte contraction induced by oxidative-nitrative stress impairs capillary reflow despite successful opening of an occluded cerebral artery. Nat. Med. 15, 1031–1037 (2009).
Google Scholar
Berthiaume, A.-A., Hartmann, D. A., Majesky, M. W., Bhat, N. R. & Shih, A. Y. Pericyte structural remodeling in cerebrovascular health and homeostasis. Front. Aging Neurosci. 10, 210 (2018).
Google Scholar
Erdener, ŞE., Küreli, G. & Dalkara, T. Contractile apparatus in CNS capillary pericytes. Neurophotonics 9, 021904 (2022).
Google Scholar
Le, D. E., Zhao, Y. & Kaul, S. Persistent coronary vasomotor tone during myocardial ischemia occurs at the capillary level and may involve pericytes. Front. Cardiovasc. Med. 9, 930492 (2022).
Google Scholar
Fu, J., Liang, H., Yuan, P., Wei, Z. & Zhong, P. Brain pericyte biology: from physiopathological mechanisms to potential therapeutic applications in ischemic stroke. Front. Cell. Neurosci. 17, 1267785 (2023).
Google Scholar
Fraser, J. F. et al. Intra-arterial verapamil post-thrombectomy is feasible, safe, and neuroprotective in stroke. J. Cereb. Blood Flow Metab. 37, 3531–3543 (2017).
Google Scholar
Nazir, S. A. et al. The REFLO-STEMI (REperfusion Facilitated by LOcal adjunctive therapy in ST-Elevation Myocardial Infarction) trial: a randomised controlled trial comparing intracoronary administration of adenosine or sodium nitroprusside with control for attenuation of microvascular obstruction during primary percutaneous coronary intervention. Efficacy Mech. Eval. https://doi.org/10.3310/eme03090 (2016).
Hill, R. A. et al. Regional blood flow in the normal and ischemic brain is controlled by arteriolar smooth muscle cell contractility and not by capillary pericytes. Neuron 87, 95–110 (2015).
Google Scholar
Hall, C. N. et al. Capillary pericytes regulate cerebral blood flow in health and disease. Nature 508, 55–60 (2014).
Google Scholar
Rangaswami, J. et al. Cardiorenal syndrome: classification, pathophysiology, diagnosis, and treatment strategies: a scientific statement from the american heart association. Circulation 139, e840–e878 (2019).
Google Scholar
Mancianti, N., Calatroni, M., Deferrari, G. & La Porta, E. Editorial: kidney and heart cross-talk. Front. Nephrol. 5, 1598135 (2025).
Google Scholar
McCullough, P. A. & Ronco, C. Heart-Kidney Cross-Talk. in Critical Care Nephrology Ch. 109, 664–670.e3 https://doi.org/10.1016/B978-0-323-44942-7.00109-6 (Elsevier, 2019).
House, A. A. et al. Heart failure in chronic kidney disease: conclusions from a Kidney Disease: Improving Global Outcomes (KDIGO) controversies conference. Kidney Int. 95, 1304–1317 (2019).
Google Scholar
Ronco, C., Bellomo, R. & Kellum, J. A. Acute kidney injury. Lancet 394, 1949–1964 (2019).
Google Scholar
Annibali, G. et al. “No-reflow” phenomenon: a contemporary review. J. Clin. Med. 11, 2233 (2022).
Google Scholar
Wanjare, M., Kusuma, S. & Gerecht, S. Perivascular cells in blood vessel regeneration. Biotechnol. J. 8, 434–447 (2013).
Google Scholar
Suthar, S. K. et al. Comprehensive SAR analysis of actomyolytics, drug candidates targeting the actomyosin complex. Eur. J. Med. Chem. 299, 117999 (2025).
Google Scholar
Gyimesi, M. et al. Single residue variation in skeletal muscle myosin enables direct and selective drug targeting for spasticity and muscle stiffness. Cell 183, 335–346.e13 (2020).
Google Scholar
Straight, A. F. et al. Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor. Science 299, 1743–1747 (2003).
Google Scholar
Allingham, J. S., Smith, R. & Rayment, I. The structural basis of blebbistatin inhibition and specificity for myosin II. Nat. Struct. Mol. Biol. 12, 378–379 (2005).
Google Scholar
Sirigu, S. et al. Highly selective inhibition of myosin motors provides the basis of potential therapeutic application. Proc. Natl Acad. Sci. USA 113, E7448–E7455 (2016).
Google Scholar
Haase, M. et al. Pathogenesis of cardiorenal syndrome type 1 in acute decompensated heart failure: workgroup statements from the eleventh consensus conference of the Acute Dialysis Quality Initiative (ADQI). Contrib. Nephrol. 182, 99–116 (2013).
Google Scholar
Virzì, G. M. et al. The hemodynamic and nonhemodynamic crosstalk in cardiorenal syndrome type 1. Cardiorenal Med. 4, 103–112 (2014).
Google Scholar
Divakaran, S. & Loscalzo, J. The role of nitroglycerin and other nitrogen oxides in cardiovascular therapeutics. J. Am. Coll. Cardiol. 70, 2393–2410 (2017).
Google Scholar
Aetesam-ur-Rahman, M. et al. Adenosine-induced coronary steal is observed in patients presenting with ST-segment–elevation myocardial infarction. J. Am. Heart Assoc. 10, e019899 (2021).
Google Scholar
Juricic, S. et al. Molecular and cellular mechanisms of myocardial ischemia and reperfusion injury: a narrative review. Cells 15, 265 (2026).
Google Scholar
Steegh, F. M. E. G. et al. Early loss of peritubular capillaries after kidney transplantation. J. Am. Soc. Nephrol. 22, 1024–1029 (2011).
Google Scholar
Ajtai, K., Mayanglambam, A., Wang, Y. & Burghardt, T. P. Human tonic and phasic smooth muscle myosin isoforms are unresponsive to the loop 1 insert. ISRN Struct. Biol. 2013, 634341 (2013).
Google Scholar
Gyimesi, M. et al. The mechanism of the reverse recovery step, phosphate release, and actin activation of Dictyostelium myosin II. J. Biol. Chem. 283, 8153–8163 (2008).
Google Scholar
Vonrhein, C. et al. Data processing and analysis with the autoPROC toolbox. Acta Crystallogr. D Biol. Crystallogr. 67, 293–302 (2011).
Google Scholar
McCoy, A. J. et al. Phaser crystallographic software. J. Appl. Crystallogr. 40, 658–674 (2007).
Google Scholar
Waterhouse, A. et al. SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46, W296–W303 (2018).
Google Scholar
Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D Biol. Crystallogr. 60, 2126–2132 (2004).
Google Scholar
Bricogne G. et al. BUSTER v.2.10.3. https://sbgrid.org/software/titles/buster (Global Phasing, 2018).
Mulvany, M. J. & Halpern, W. Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ. Res. 41, 19–26 (1977).
Google Scholar
Mastitskaya, S., de Freitas, F. S. S., Evans, L. E. & Attwell, D. GLP-1 activates KATP channels in coronary pericytes as the effector of brain-gut-heart signalling mediating cardioprotection. Nat. Commun. 17, 2773 (2026).
Google Scholar
Besseling, P. J. et al. A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats. Am. J. Physiol. Renal Physiol. 320, F518–F524 (2021).
Google Scholar
Seylaz, J. et al. Dynamic in vivo measurement of erythrocyte velocity and flow in capillaries and of microvessel diameter in the rat brain by confocal laser microscopy. J. Cereb. Blood Flow Metab. 19, 863–870 (1999).
Google Scholar
Liebschner, D. et al. Polder maps: improving OMIT maps by excluding bulk solvent. Acta Crystallogr. Struct. Biol. 73, 148–157 (2017).
Google Scholar
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