Cell culture HCT116 cells and derivative cell lines (Supplementary Table 1) were grown in DMEM (Life Technologies, 41966-029) supplemented with 10% fetal bovine serum (FBS, GIBCO), 100 U ml−1 penicillin and 100 mg ml−1 streptomycin in an atmosphere containing 6% CO2 at 37 °C. HCT116 OsTIR (F74G), mAID-mClover-RAD21 HCT116 OsTIR (F74G)6, WAPL-mClover-mAID HCT116 OsTIR (F74G)4 and CTCF-mClover-mAID HCT116 OsTIR (F74G)6
The world governing body had also previously informed the ECB that any footage filmed at the PMOA must not contain audio or be released before the conclusion of a match. Speaking at the end of day four, Stokes explained why the public announcement was made during the game, detailing that it was a plan between
