Training of snTIMs PsychAD cohort Molecular profiling efforts of the PsychAD consortium include snRNA-seq from more than 6 million nuclei from the DLPFC of 1,494 unique donors15. As described in the Capstone paper11, we utilized quadratic discriminant analysis using the 1000 Genomes Project58,59 reference to divide the full cohort into five discrete superpopulations based on
Collection and harmonization of clinical, pathological and demographic metadata Brain tissue specimens were sourced from two brain banks: the Mount Sinai NIH Neurobiobank (MSSM) (1,042 samples) and the NIMH-IRP Human Brain Collection Core (HBCC) (300 samples). Furthermore, samples were obtained from five prospective cohort studies conducted at the Rush Alzheimer’s Disease Center (RADC) (152 samples)124,125.
Mice All mice were group-housed and experiments were conducted in accordance with procedures approved by the Institutional Animal Care and Use Committees at the Massachusetts General Hospital and the Tufts University School of Medicine and NIH guidelines. All mice were housed in a 12-h (7:00 to 19:00) light–dark colony room at 22–24 °C, 30–50% humidity and
