Expression and purification of SC003-mi3 VLP The mi3-VLP (also known as SC003-mi3) was derived from i3-01 (ref. 66) by introducing two mutations that remove surface-exposed cysteines to prevent aggregation and by genetically fusing a SpyCatcher domain to the N-terminus to enable plug-and-play attachment of SpyTagged proteins54. The plasmid pET28a-SpyCatcher003(SC003)-mi3 (gift from Professor Mark Howarth, Cambridge University)
Experimental setup A detailed description of our experimental setup has been given in previous works35,60,61. In short, we trap individual 88Sr atoms in a programmable one-dimensional array of optical tweezers (813 nm) generated by acousto-optic deflectors (AODs). The atoms are initialized in the 5s2 1S0 state and cooled on the narrow-line red transition 5s2 1S0 ↔ 5s5p 3P1
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