Cell culture MCF7 (HTB-22), ZR-75-1 (CRL-1500), CAMA-1 (HTB-21), MDA-MB-453 (HTB-131), MDA-MB-231 (HTB-26), OVCAR-3 (HTB-161) and Kuramochi (JCRB0098) cells were obtained from the American Type Culture Collection. MCF7 ESR1Y537S cells were provided by S. Chandarlaparty35. MCF7, ZR-75-1, MDA-MB-231 and CAMA-1 cells were cultured in RPMI-1640 medium (11875-093, Gibco) supplemented with 10% fetal bovine serum (FBS; SH30054.03,
Plasmids and inserts Sequences and accompanying information are given in Supplementary Tables 2–4. In brief, we selected Codebook TFs (and their DBDs) from information published in a previous study1 and posted at https://humantfs.ccbr.utoronto.ca. Inserts named with a ‘-FL’ suffix correspond to the full-length ORF of a representative isoform of the protein. Those with a ‘-DBD’
I constantly use dictation apps such as Wispr Flow, Willow, or Typeless to reply to messages and emails on both my Mac and my phone. But to do so, I have to use my phone’s mic or AirPods to dictate my messages, and they often don’t pick up what I am saying. That’s why when
