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Bacterial strains All strains for the experiments were grown in lysogeny broth (LB) Lennox and the required antibiotics and incubated at 37 °C with shaking. E. coli K-12 (BW25113)72 was used for most biological analyses, and the BL21-AI or NEB5α strains were used for protein purification and cloning, respectively. The use of the ECOREF53 strain collection
Details of observations Our observations, from which we inferred S301, comprise 19 datasets spanning more than 8 years. We first noted S301 in 2023, when pointing directly to Sgr A*. Given the position of the star close to Sgr A* and its large proper motion, it was clear that it might be on a tight orbit,