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tRNA in vitro production Oligonucleotides containing tRNA under a T7 promoter and followed by an HDV ribozyme61 were synthesized by IDT as high-fidelity DNA microchip oligo pools from 254–275 nucleotides in length, and dsDNA was amplified in a single PCR reaction for each pool. These tRNA pools included 48 unique E. coli isoacceptor tRNAs with
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