Fly stocks and husbandry Flies were housed at 25 °C and 50–65% relative humidity on a 12 h light/12 h dark cycle, except for animals used in optogenetics experiments, which were reared in darkness. Stocks used for behavioural experiments were reared on standard molasses food, 2P imaging lines were reared on Würzburg food for at least five generations
Peters, A. J., Chen, S. X. & Komiyama, T. Emergence of reproducible spatiotemporal activity during motor learning. Nature 510, 263–267 (2014). Article ADS CAS PubMed Google Scholar Guo, L. et al. Dynamic rewiring of neural circuits in the motor cortex in mouse models of Parkinson’s disease. Nat. Neurosci. 18, 1299–1309 (2015). Article CAS PubMed PubMed
Mice All mouse handling and maintenance was performed according to the regulations of the Institutional Animal Care and Use Committee of the Albert Einstein College of Medicine (protocol 00001393). Sstflp+/+; Nos1creER+/− and Sstflp+/−;Nos1creER+/− mice were used interchangeably in this study, with no differences detected between the two groups (Nos1creER, Jax 014541; Sstflp, Jax 031629). Nos1creER+/−
Devane, W. A., Dysarz, F. A., Johnson, M. R., Melvin, L. S. & Howlett, A. C. Determination and characterization of a cannabinoid receptor in rat brain. Mol. Pharmacol. 34, 605–613 (1988). Article CAS PubMed Google Scholar Herkenham, M. et al. Cannabinoid receptor localization in brain. Proc. Natl Acad. Sci. USA 87, 1932–1936 (1990). Article ADS
Bernardi, S. et al. The geometry of abstraction in the hippocampus and prefrontal cortex. Cell 183, 954–967 (2020). Article CAS PubMed PubMed Central Google Scholar Gallego, J. A., Perich, M. G., Miller, L. E. & Solla, S. A. Neural manifolds for the control of movement. Neuron 94, 978–984 (2017). Article CAS PubMed PubMed Central Google
Mice Mice were maintained on a 12 h:12 h light:dark cycle with ad libitum access to food and water, at 22 ± 2 °C and 55 ± 10% humidity. All experiments were performed in accordance with the Swiss National Institutional Guidelines on Animal Experimentation and were approved by the cantonal Veterinary Office Committees for Animal Experimentation. Male C57BL6/J mice were used for
