Cell culture and nucleofection K562 (CCL-243) and Jurkat (TIB-152) cells were obtained from American Type Culture Collection (ATCC) and cultured at 37 °C under 5% CO2 in RPMI media (ThermoFisher Scientific, 11875093) supplemented with 10% FBS, and 1% Penicillin/Streptomycin (ThermoFisher Scientific, 15140122). HEK293T (CRL-1573) cells were obtained from the ATCC and cultured at 37 °C under 5%
International students, so-called “visa hoppers” and backpackers will be targeted under stricter rules announced by Australia on Thursday in a bid to curb immigration. Foreign students will no longer be able to bring their families with them and those who want to extend their stay must choose a higher qualification. Rules will also be stricter
Giving an answer to a question that is asked at a free-flowing press conference is one thing, but Donald Trump repeated his sentiments a short time later when he was delivering a speech to an invited audience at the US Ambassador’s residence. In fact it was almost the first thing he mentioned, hinting that maybe
Attacks on military and defence targets are not new, although previous acts of sabotage linked to Russia include more puzzling targets – such as an Ikea store and a Polish paint store. The novelty now is the concentration of cases. “The intensity of military sites being attacked we’ve seen over the past couple of weeks
The US military says it has hit three Iran-linked oil tankers after ballistic missiles were launched towards two of its warships in the region. US Central Command (Centcom) said on Saturday it had “permanently disabled” a tanker near Kharg Island and another near the Strait of Hormuz, and “completely destroyed” a third in the Gulf
Synthesis and isolation of all related compounds All available compounds were purchased from commercial suppliers around the world. All solvents for synthesis were purchased from local suppliers, and solvents of high-performance liquid chromatography (HPLC)-grade were purchased from Fisher Scientific. The synthetic routes and chemicals used for all related compounds are shown in Supplementary Information section 2
