Labelling the cellular structures with ECL probes Preparation of Ru(bpy)3 2+–N-hydroxysuccinimide-ester-labelled antibody 1 ml, 1 mg ml−1 goat anti-rabbit immunoglobulin G (IgG) antibody (Huabio, HA1002) was dialysed (Viskase, Membra-Cel MD44) overnight at 4 °C in 3 l of 0.01 M phosphate buffer saline (PBS, pH 7.6, Sigma) and adjusted to a concentration of 2 mg ml−1. Then 1 mg of Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 (Aladdin, R131404) was dissolved
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Gordon, J. et al. The road towards understanding embodied decisions. Neurosci. Biobehav. Rev. 131, 722–736 (2021). Article PubMed PubMed Central Google Scholar Maselli, A. et al. Beyond simple laboratory studies: developing sophisticated models to study rich behavior. Phys. Life Rev. 46, 220–244 (2023). Article ADS PubMed Google Scholar Yoo, S. B. M., Hayden, B. Y.
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Aleixo, I. et al. Amazonian rainforest tree mortality driven by climate and functional traits. Nat. Clim. Change 9, 384–388 (2019). Article ADS Google Scholar Uriarte, M., Lasky, J. R., Boukili, V. K. & Chazdon, R. L. A trait-mediated, neighbourhood approach to quantify climate impacts on successional dynamics of tropical rainforests. Funct. Ecol. 30, 157–167 (2016).
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Explanation: Known for its bright and fast meteors, the annual Perseid Meteor Shower comes to planet Earth’s skies from a radiant in the heroic constellation Perseus. The popular northern summer celestial spectacle is created as grains of dust cast off along the orbit of periodic comet 109P/Swift-Tuttle vaporize in Earth’s dense atmosphere, tracing brief, but
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Children in the United States normally get two doses of the MMR vaccine: one at 12 to 15 months, and one at 4 to 6 years of age.Credit: Courtney Perry/Washington Post/Getty Researchers are worried that an executive order signed by US President Donald Trump earlier this week will have negative consequences for children’s health across
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Fauset, S. et al. Drought-induced shifts in the floristic and functional composition of tropical forests in Ghana. Ecol. Lett. 15, 1120–1129 (2012). Article PubMed Google Scholar Lewis, S. L. et al. Above-ground biomass and structure of 260 African tropical forests. Philos. Trans. R. Soc. B 368, 20120295 (2013). Article Google Scholar Álvarez-Dávila, E. et al.
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