Youle, R. J. & Narendra, D. P. Mechanisms of mitophagy. Nat. Rev. Mol. Cell Biol. 12, 9–14 (2011). Article CAS PubMed PubMed Central Google Scholar Mizushima, N., Levine, B., Cuervo, A. M. & Klionsky, D. J. Autophagy fights disease through cellular self-digestion. Nature 451, 1069–1075 (2008). Article CAS PubMed ADS PubMed Central Google Scholar Jiao,
The protein catalogue gives a dynamic picture of the mitochondria, beyond what was known from humans and yeast. Credit: Dr David Furness/Keele University/SPL Scientists have mapped in unprecedented detail the proteins that make up the mitochondria in six organisms — five single-celled parasites and one plant — spanning the eukaryotic tree of life. The findings,
A microscope image of a mitochondrion, artificially coloured in green and red. The organelles were extracted for a retinal transplant from the person’s own leg muscles. Credit: K. R. Porter/SPL Scientists have extracted mitochondria from a woman’s leg muscles and injected them into the vitreous fluid of her eyes in an attempt to treat severe
Labelling the cellular structures with ECL probes Preparation of Ru(bpy)3 2+–N-hydroxysuccinimide-ester-labelled antibody 1 ml, 1 mg ml−1 goat anti-rabbit immunoglobulin G (IgG) antibody (Huabio, HA1002) was dialysed (Viskase, Membra-Cel MD44) overnight at 4 °C in 3 l of 0.01 M phosphate buffer saline (PBS, pH 7.6, Sigma) and adjusted to a concentration of 2 mg ml−1. Then 1 mg of Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 (Aladdin, R131404) was dissolved
A needle approaches an egg (purple) during in vitro fertilization, which some clinics offer in combination with a procedure called mitochondrial replacement therapy. Credit: Zephyr/Scientific Photo Library A small number of fertility clinics around the world are offering a controversial treatment: a procedure to provide clients’ embryos with donated mitochondria as a way to increase
