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Antiproton reservoir preparation The BASE-STEP catching trap is constructed of 15 cylindrical electrodes arranged in a coaxial stack (Extended Data Fig. 1a). Sapphire rings ensure electrical insulation and mechanical alignment. Antiprotons emerging from the degrader foil follow the magnetic field lines (B = 993 mT) into the trap, where three high-voltage electrodes (C01–C03) on the degrader side capture
Cell culture HCT116 cells and derivative cell lines (Supplementary Table 1) were grown in DMEM (Life Technologies, 41966-029) supplemented with 10% fetal bovine serum (FBS, GIBCO), 100 U ml−1 penicillin and 100 mg ml−1 streptomycin in an atmosphere containing 6% CO2 at 37 °C. HCT116 OsTIR (F74G), mAID-mClover-RAD21 HCT116 OsTIR (F74G)6, WAPL-mClover-mAID HCT116 OsTIR (F74G)4 and CTCF-mClover-mAID HCT116 OsTIR (F74G)6
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