Clinical trial design, patients and conduct The Algonquin study is an ongoing, multicentre, open-label, single-arm, two-part study (ClinicalTrials.gov identifier: NCT03715478)) of BPd in patients with relapsed/refractory MM. Part 1 of the trial consisted of a dose-exploration phase22. Part 2 is evaluating the safety, tolerability and clinical activity of the dose and schedule identified in part 1. Patients included
Expression and purification of SC003-mi3 VLP The mi3-VLP (also known as SC003-mi3) was derived from i3-01 (ref. 66) by introducing two mutations that remove surface-exposed cysteines to prevent aggregation and by genetically fusing a SpyCatcher domain to the N-terminus to enable plug-and-play attachment of SpyTagged proteins54. The plasmid pET28a-SpyCatcher003(SC003)-mi3 (gift from Professor Mark Howarth, Cambridge University)
This interview has been edited for length and clarity. JORGE GARAY: AI BioDesign seeks to explore possible molecules and biological functions that have never existed in nature. Are there any particular risks involved in venturing into this uncharted territory? How can scientists anticipate those risks and what steps can they take to minimize them before
Genetically encoded cortical depletion Emx1-cre (JAX, B6.129S2-Emx1tm1(cre)Krj/J), Esco2fl/fl (JAX, B6N.129S-Esco2tm1.1Ge/J) and C57BL SCID (JAX, B6.Cg-Prkdcscid/SzJ) mice were used to generate the breeding scheme: male Esco2fl/flPrkdcscid/scid × female Emx1-cre+/−Esco2fl/+Prkdcscid/scid. Breeder and offspring cages were supplemented with breeder chow (Envigo Teklad 2919) and DietGel 76A (with plant protein) to help to increase pup survival. Some breeders were more prone to
ChEMBL database molecular pairs From the PostgreSQL version of CHEMBL34 (ref. 4) (https://doi.org/10.6019/CHEMBL.database.34), we filtered the database for compounds with activities reported against a single protein, with either Ki, Kd, IC50 or EC50 as the activity type. To be considered a molecular pair, compounds had to have the same assay ID, the same target ID,
Riemensberger, J. et al. A photonic integrated continuous-travelling-wave parametric amplifier. Nature 612, 56–61 (2022). Article ADS CAS PubMed Google Scholar Tran, M. et al. Extending the spectrum of fully integrated photonics. Nature 610, 54–60 (2022). Article ADS CAS PubMed PubMed Central Google Scholar Kaur, P. et al. Hybrid and heterogeneous photonic integration. APL Photonics 6,
