All surgical and experimental procedures were in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committees of the Allen Institute or Johns Hopkins University. Allocations of mice to experiments were randomized and experimenter blind. Mice were housed at 20–22 °C
Patient recruitment and sample collection Specimens were obtained from 20 patients, including histopathologically normal tissue (n = 20, flash-frozen tissue), primary tumour samples (n = 24; 16 FFPE and 8 flash-frozen tissue) and 1–7 metastases per patient (n = 80, flash-frozen tissue). Details of tissue source, location, histology, among other information, for each sample are provided in Supplementary Table 1. Autopsy
Biosafety and biosecurity We performed all experiments with pseudotyped lentiviral particles at biosafety level 2 (ref. 59). These pseudoviruses do not encode any other viral proteins other than RSV F and therefore can only undergo a single round of cell entry, and so they are not fully replicative infectious agents capable of causing disease. The other
Experimental apparatus and VUV beam guiding The design of the spectroscopy apparatus is shown in Extended Data Fig. 1. It consists of the chamber with the SBO crystal for SHG frequency conversion, a vacuum beamline, a VUV spectrometer and a spectroscopy chamber with a thorium-doped crystal and the detection system. The generated VUV beam is aligned
Details on implementing Paper2Agent Paper2Agent converts a research paper and its public codebase into a production-ready MCP server and then exposes that server to an AI agent interface. We implemented this as a multi-agent system using Claude Code’s agent SDK, where a central orchestrator agent coordinates specialized sub-agents through a six-step pipeline. Each sub-agent is
