Study design The samples for this study were obtained from the Lifelines NEXT (LLNEXT) cohort, a birth cohort designed to study the effects of intrinsic and extrinsic predictors of health and disease in a four-generation design23. LLNEXT is embedded within the Lifelines (LL) cohort study, a prospective three-generation population-based cohort study recording the health and
Cell culture MCF7 (HTB-22), ZR-75-1 (CRL-1500), CAMA-1 (HTB-21), MDA-MB-453 (HTB-131), MDA-MB-231 (HTB-26), OVCAR-3 (HTB-161) and Kuramochi (JCRB0098) cells were obtained from the American Type Culture Collection. MCF7 ESR1Y537S cells were provided by S. Chandarlaparty35. MCF7, ZR-75-1, MDA-MB-231 and CAMA-1 cells were cultured in RPMI-1640 medium (11875-093, Gibco) supplemented with 10% fetal bovine serum (FBS; SH30054.03,
A woman identified as Jane Doe 4 has joined a lawsuit filed by three Tennessee teenagers against Elon Musk’s xAI over the role the company’s chatbot Grok allegedly played in creating child sexual abuse material. According to a report in The Washington Post, the woman alleged that her stepfather used Grok to manipulate a photo
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Maintenance of mouse lines All animal procedures were approved by the Austrian Federal Ministry of Women, Science and Research in accordance with the Austrian and European Union animal law (license number: BMWF-66.018/0007-II/3b/2012; BMWFW-66.018/0006-WF/V/3b/2017; GZ: 2020-0.579.989 and GZ: 2025-0.597.515). Experimental mice were bred and maintained according to regulations approved by the institutional animal care and use committee and