Inhibitors for in vitro and cell-based assays were obtained as follows: ponatinib (MedChemExpress (MCE), HY-12047); asciminib (MCE, HY-104010); imatinib (MCE, HY-15463); dasatinib (MCE, HY-10181); osimertinib (MCE, HY-15772); and RMC-5552 (MCE, HY-132168). DasatiLink-1 and asc-tracer were obtained from the same batches as previously described14,34. DAS-DFGO-II and DAS-CHO-II were provided by M. Soellner. Das-tracer44 was provided by Promega. Synthesis
Human samples Snap-frozen and formalin-fixed paraffin-embedded (FFPE) liver tissue sections from de-identified patients with PDAC and individuals without PDAC were obtained from the Rapid Autopsy Program at the University of Nebraska Medical Center (UNMC), in accordance with institutional review board (IRB) approval (IRB 091-01). Written informed consent for the research use of these specimens was
Ethical approval Before commencement of the analysis, approval was obtained following review by the Privacy Risk and Impact Assessment (PRIA) governance body of the bank. Following an initial review by a Data Privacy Manager and a Data Privacy Risk Specialist, the application underwent an additional review by a Group Data Protection Officer, in accordance with
Materials Chemicals were purchased from commercial suppliers (Thermo Scientific, Merck KGaA, VWR and Honeywell). Solvents and reagents used for peptide synthesis were obtained in peptide grade. Solvents used for peptide purification were of high-performance liquid chromatography (HPLC) grade; dimethyl sulfoxide (DMSO) was BioUltra grade for molecular biology (≥99.5%, Sigma Aldrich); and water was obtained from
Experimental sequence Each interrogation beginsonly after both ions have been subjected to Doppler cooling and prepared in the |g⟩ state with >99% probability using the conditional state preparation sequence described in ref. 53. Both ions are interrogated with the HA–HR sequence shown in Fig. 1d, with the parameters τL ≈ 4 ms, τ1 = τ2 ≈ 12 ms, and a total Ramsey time given
Training of snTIMs PsychAD cohort Molecular profiling efforts of the PsychAD consortium include snRNA-seq from more than 6 million nuclei from the DLPFC of 1,494 unique donors15. As described in the Capstone paper11, we utilized quadratic discriminant analysis using the 1000 Genomes Project58,59 reference to divide the full cohort into five discrete superpopulations based on
